Part:BBa_K5401005

T7 RNA polymerase (T7RNAP) antisense oligonucleotide (ASO)

The antisense oligonucleotide is a short RNA (sRNA) sequence complementary to the T7RNAP mRNA sequence. It binds to +4 to +27 of the mRNA sequence, thereby inhibiting translation and regulating the translation of T7RNAP.

The DNA sequence provided transcripts an sRNA sequence complementary to the wild-type T7RNAP (BBa_J435007).

Usage and Biology

The aim of the antisense oligonucleotide is to bind to the T7RNAP mRNA and inhibit the translation.

In our design: the Hfq protein binds to sRNAs (an antisense oligonucleotide targeted against T7RNAP mRNA), thereby helping them to recognize and bind to complementary regions on mRNAs. This binding blocks ribosome access to the mRNA's start codon or Shine-Dalgarno sequence, thus inhibiting translation. This helps to knockdown the expression of T7RNAP, thereby regulating the expression of T7RNAP.

Characterization

This part, together with a Hfq scaffold sequence, and a lac promoter acts to regulate the expression of T7RNAP, thereby allowing a stable expression.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]