Plasmid

Part:BBa_K5302021

Designed by: Xinyuan Shi   Group: iGEM24_USTC   (2024-10-01)
Revision as of 14:29, 1 October 2024 by Hagibis (Talk | contribs)


8IJZ

This work is derived from pBBR1MCS-OmpA-mCherry and pUC19-miniZ-Z3C, and it has undergone codon optimization. This composite part combines OmpA(21.4kda) and miniZ(3.8kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express miniZ. The plasmid uses lac promotor and has kanamycin resistence.

Jamboree Program
Figure 1. Colony PCR results of pBBR-OmpA-miniZ

Jamboree Program
Figure 2. SDS-PAGE analysis of pBBR1MCS-OmpA-miniZ expression in Escherichia coli Nissle 1917 （1）

Jamboree Program
Figure 3. SDS-PAGE analysis of pBBR1MCS-OmpA-miniZ expression in Escherichia coli Nissle 1917 （2）

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//plasmid
Parameters
biologyEscherichia coli Nissle 1917