Part:BBa_K5108004

Creatinine amidohydrolase form Pseudomonas putida

Creatinine amidohydrolase from Pseudomonas putida

Contents
1. Usage and Biology
2. References

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 61
  Illegal NgoMIV site found at 663
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 199
  Illegal BsaI.rc site found at 547
  

Usage and Biology

Creatinine amidohydrolase (CrnA) is an enzyme that catalyzes the degradation of creatinine into creatine in Pseudomonas putida. In the context of our project, the gene encoding this enzyme (crnA) was synthesized in an operon together with the gene for creatine degradation. You can find more informations about construct and results in BBa_K5108009. SDS-PAGE, growth analysis and consumption analysis of creatinine by NMR spectroscopy were performed. Figure 1 displays the intermediate step of construction.

Figure 1: Design of the expression plasmid harboring the crnA gene.

References

UniProt. (s. d.). https://www.uniprot.org/uniprotkb/P83772/entry

Tsuru, D., Oka, I., & Yoshimoto, T. (1976c). Creatinine Decomposing Enzymes inPseudomonas putida. Agricultural And Biological Chemistry, 40(5), 1011‑1018. https://doi.org/10.1080/00021369.1976.10862151