Part:BBa_K5108003

Creatinase from Pseudomonas putida

Creatinase from Pseudomonas putida

Contents
1. Usage and Biology
2. References

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 225
  Illegal BsaI site found at 1012
  

Usage and Biology

Creatinase (CreA) is an enzyme that catalyzes the degradation of creatine into sarcosine in Pseudomonas putida. In the context of our project, the gene encoding this enzyme (creA) was synthesized in an operon together with the gene for creatine degradation. You can find more informations about construct and results in BBa_K5108009. SDS-PAGE, growth analysis and consumption analysis of creatinine by NMR spectroscopy were performed. Figure 1 displays the intermediate step of construction.

Figure 1: Design of the expression plasmid harboring the creA gene.

References

UniProt. (s. d.-b). https://www.uniprot.org/uniprotkb/P38488/entry

Cloning, Expression and Purification of Pseudomonas putida ATCC12633 Creatinase. (2017, 1 décembre). PubMed. https://pubmed.ncbi.nlm.nih.gov/29090065/