Coding
pelA

Part:BBa_K5193001

Designed by: CHIEN-YUEH LIU   Group: iGEM24_PuiChing-Macau   (2024-09-15)
Revision as of 08:10, 15 September 2024 by Registry (Talk | contribs)

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pelA (Pectrobacterium astrospecticum)

This is a pectinase from Pectrobacterium astrospecticum with no thermostable ability. In the part design, we added a NSP4 tag before the sequence to enhance secretion of the protein. We used it to hydrolyze pectin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 503
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 664
    Illegal AgeI site found at 997
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None