Composite
Part:BBa_K5226077
Designed by: Yujiao Yang Group: iGEM24_SCUT-China-A (2024-10-01)
gRNA-△cysNC-key
1
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1852
Illegal XbaI site found at 98
Illegal PstI site found at 1474
Illegal PstI site found at 2047 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1852
Illegal NheI site found at 765
Illegal PstI site found at 1474
Illegal PstI site found at 2047 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1852
Illegal BamHI site found at 92
Illegal BamHI site found at 1002 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1852
Illegal XbaI site found at 98
Illegal PstI site found at 1474
Illegal PstI site found at 2047 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1852
Illegal XbaI site found at 98
Illegal PstI site found at 1474
Illegal PstI site found at 2047
Illegal NgoMIV site found at 364 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |