Part:BBa_K5291003

vgb: Vitreoscilla hemoglobin

vgb encodes VHb. VHb, the first discovered bacterial hemoglobin, is a soluble heme-binding protein with a faster rate of oxygen dissociation. It can enhance cell growth, product synthesis and stress tolerance. Especially under oxygen-limited conditions, VHb can interact with terminal oxidase to deliver enough oxygen to improve the degradation ability of CYPY96F on PE.

Usage and Biology

In degradation module we constructed pAB1-CYPY96F-VHB to ensure the expression of emzymes related to degradation.

Fig.1 The map of plasmid pAB1-CYPY96F-VHB.

Moreover,CYPY96F proteins was successfully expressed in the strains. SDS-PAGE was performed and the following results were obtained.

Fig.2 The SDS-PAGE result of CYPY96F.

Since we set CYPY96F and VHB as a whole, the overall functions need moving to the part BBa_K5291034.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]