Part:BBa_K5407001

si-TEAD4-1 sequence

Usage and Biology

The HIPPO pathway is a key regulatory pathway in tumor development, with TEAD4 (TEA Domain Family Member 4) being a core transcription factor that promotes the growth and spread of CRC cancer cells [1]. si-TEAD4-1 sequence are designed to target to the untranslated region (UTR) of TEAD4. When siRNA reach the cell cytoplasm, it could binds with the RNA-induced silencing complex (RISC) [2]. Binding with the RISC leads to degradation of the sense strand, whereas the antisense strand continues to be incorporated in the RISC [3]. The RISC then makes multiple mRNA cleavages to downregulate TEAD4 gene expression.

Design

We designed the siRNA against TEAD4 by a special database (https://www.invivogen.com/sirnawizard/). After we input the TEAD4 gene name and sequence, the database would output all the possible siRNA sequence (Figure 1). We chose two pairs of siRNAs with the least off-target possibility.

                                
                              Figure 1 Design of siRNA Sequences Targeting TEAD4 

Reference

Reference

[1] Evelien, Dekker,Pieter J, Tanis,Jasper L A, Vleugels et al. Colorectal cancer.[J] .Lancet, 2019, 394: 0.

[2] Shigeyoshi, Kijima,Takahiro, Sasaki,Koichi, Nagata et al. Preoperative evaluation of colorectal cancer using CT colonography, MRI, and PET/CT.[J] .World J Gastroenterol, 2014, 20: 0.

[3] Robert H, Hollis,Daniel I, Chu,Healthcare Disparities and Colorectal Cancer.[J] .Surg Oncol Clin N Am, 2022, 31: 0.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]