Coding

Part:BBa_K5302002

Designed by: Jiacan Ma   Group: iGEM24_USTC   (2024-09-30)
Revision as of 20:44, 30 September 2024 by Registry (Talk | contribs)

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8IJZ

This year, the USTC iGEM team has utilized the competitive binding of vascular endothelial growth factor (VEGF) to develop a targeted bacterial therapy for solid tumors. Our quest for the optimal VEGF-binding protein(or peptide) led us to an in-depth exploration of proteins structurally akin to the vascular endothelial growth factor receptor (VEGFR), which we have named VEGFR-like. 8IJZ is an anti-VEGF nanobody, and it has 2 chains.These two chains all show great affinity with VEGF, we plan to use pBBR1MCS-2 plasmid as a backbone and transfer 8IJZ into Escherichia coli Nissle 1917, and finally succeeded in expressing 8IJZ.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/receptor
Parameters
biologyEscherichia coli Nissle 1917