Composite

Part:BBa_K5301019

Designed by: Xing Zhang   Group: iGEM24_BNU-China   (2024-09-24)
Revision as of 07:56, 1 October 2024 by Furina1013 (Talk | contribs)


MSP1E3D1 expression pathway (T7) This pathway expresses MSP1E3D1 using T7 expression system.

Description

We used this pathway to express MSP1E3D1 by using T7 expression system in E.coli, and the expression of MSP1E3D1 can be induced by IPTG. For more information on MSP1E3D1, go to BBa_K5301000.
Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 173
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 173
    Illegal NheI site found at 903
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 173
    Illegal BglII site found at 799
    Illegal XhoI site found at 695
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 173
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 173
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None