Device

Part:BBa_K239015:Experience

Designed by: Axel Nystrom   Group: iGEM09_UCL_London   (2009-10-19)
Revision as of 02:08, 22 October 2009 by Zcbcd06 (Talk | contribs) (→‎User Reviews)

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Applications of BBa_K239015

Test device for BBa_K239000. Designed to detect misfolding of proteins in the periplasm or shear stress. Is activated by in stationary phase, organic chemicals like Toluene, 1-octanol, and by metals such as Copper and Iron, which can cause protein misfolding in E.coli cells.

User Reviews

UNIQac6fb6e0cd8ed1cc-partinfo-00000000-QINU UNIQac6fb6e0cd8ed1cc-partinfo-00000001-QINU

The DegP promoter is dependent of Sigma E (and hence also the growth phase) and can not be induced only by over expression of e.g. CpxR. In comparison, the related Spy promoter (BBa_K239001), which is dependent on sigma factor 70, can be induced up to 40 fold by over expression of CpxR. (Bury-Moné et al. 2009) Experiments by the The UCL_London_2009 team have also showed that the activity of the DegP promoter, compared to the spy promoter, is much more dependent of the growth phase of the bacteria.

For detailed information please visit [http://2009.igem.org/Team:UCL_London/From_the_lab/Expts UCL_London Wet Lab Experiments] &[http://2009.igem.org/Team:UCL_London/From_the_lab/Results UCL_London Wet Lab Results]