Composite
Part:BBa_K5115013
Designed by: Liyue Chen Group: iGEM24_Fudan (2024-09-18)
ribozyme+RBS+hoxY+stem-loop
Introduction
This composite part is composed of hoxY and improved ribozyme-assisted polycistronic co-expression system: pRAP. The hoxY is a subunit of the hydrogenase made up by hox and hyp, get details about hoxY in BBa_K5115020(hox and hyp operon). This composite part will finally be used in BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module)for further experiment.
Usage and Biology
The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check part wiki of 2022 Fudan iGEM.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 132
Illegal XhoI site found at 339 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 432
- 1000COMPATIBLE WITH RFC[1000]
References
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Categories
Parameters
None |