Part:BBa_K5115012

ribozyme+RBS+hoxU+stem-loop

Introduction

This composite part is composed of hoxU and improved ribozyme-assisted polycistronic co-expression system: pRAP. The hoxU is a subunit of the hydrogenase made up by hox and hyp, get details about hoxU in BBa_K5115020(hox and hyp operon). This composite part will finally be used in BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module)for further experiment.

Usage and Biology

The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check part wiki of 2022 Fudan iGEM.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 485
  Illegal BglII site found at 563
  Illegal XhoI site found at 493
  Illegal XhoI site found at 685
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 446
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 267
  Illegal BsaI site found at 429
  Illegal SapI.rc site found at 378