Composite

Part:BBa_K5186008

Designed by: Jiawen Chen   Group: iGEM24_AIS-China   (2024-09-24)
Revision as of 11:17, 24 September 2024 by Registry (Talk | contribs)

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PTac-riboJ-DXS-IspG-IspDF-B0015

PTac-riboJ-DXS-IspG-IspDF-B0015 is an expression cassette in E. coli expressing DXS (BBa_K3166061), IspG (BBa_K1653001) and IspDF (BBa_K1653001) used to overexpress methylerythritol phosphate(MEP) pathway. With this expression cassette, DXS, IspG and IspDF can be IPTG-inducibly expressed under the control of pTac-RiboJ (BBa_K3552015) in all strains of E. coli. This is the best part of the MEP overexpression cassettes in the part collection where we enable the overproduction of HMBPP. The part collection includes sRNA(IspH)s (BBa_K5186001, BBa_K5186002, BBa_K5186003, BBa_K5186004, BBa_K5186005) for the downregulation of downstream gene IspH expression and various MEP overexpression cassettes (BBa_K5186006, BBa_K5186007, BBa_K5186008, BBa_K5186009). This collection can help and inspire other iGEM teams and researchers to achieve higher yield of HMBPP or other isoprenoids in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4325
    Illegal BglII site found at 5541
    Illegal BamHI site found at 3653
    Illegal BamHI site found at 4869
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2102


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Parameters
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