Part:BBa_K5115035
ribozyme+RBS+MTA+stem-loop
Introduction
This composite part is composed of MTA and improved ribozyme-assisted polycistronic co-expression system：pRAP. We introduced this ribozyme-assisted polycistronic co-expression system in 2022. By inserting ribozyme sequences before MTA, the RNA sequences of Twister ribozyme conduct self-cleaving in the mRNA..[1] To protect the mono-cistron mRNA from degradation, a stem-loop structure is placed at the 3' end of MTA.[2] The Metallothioneins (MTA) are intracellular, low molecular, low molecular weight, cysteine-rich proteins. Ubiquitous in eukaryotes, MTA have unique structural characteristics to give potent metal-binding and redox capabilities.[3]
Usage and Biology
The heterologously expressed codon-optimized MTA can endowing E.coli with detoxifying capability.
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Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 198
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Eiler, D., Wang, J., & Steitz, T. A. (2014). Structural basis for the fast self-cleavage reaction catalyzed by the twister ribozyme. Proceedings of the National Academy of Sciences, 111(36), 13028–13033.
- ↑ Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic Co-Expression. ACS Synthetic Biology, 12(1), 136–143.
- ↑ Coyle, P., Philcox, J. C., Carey, L. C., & Rofe, A. M. (2002). Metallothionein: The multipurpose protein. Cellular and Molecular Life Sciences: CMLS, 59(4), 627–647.
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