Composite

Part:BBa_K5236017

Designed by: Hening Guo   Group: iGEM24_BASIS-China   (2024-10-01)
Revision as of 02:37, 1 October 2024 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pET28a-pelB-IsPETase(M128L)-6xHis

T7 promoter: used to regulate gene expression of recombinant proteins. Lac operator: used to control gene expression by lactose. RBS: the location to bind ribosome. IsPETase M128L: This sequence encodes for a mutated IsPETase M128L and is derived from Escherichia coli. pelB: the sequence codes for signal peptide which helps PETase exit the cell membrane. 6xHis: a tag for the purification of protein which focus in the production of recombinant proteins. T7 terminator: terminates transcription.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005


[edit]
Categories
Parameters
None