Composite

Part:BBa_K5236016

Designed by: Hening Guo, Yangzihan Chu   Group: iGEM24_BASIS-China   (2024-10-01)
Revision as of 02:38, 1 October 2024 by Registry (Talk | contribs)

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pET28a-pelB-IsPETase(H104W)-6xHis

T7 promoter: A Strong promoter recognized by T7 RNA polymerase, used to regulate gene expression of recombinant proteins. Lac operator: operator that can be activated by IPTG, used to control gene expression by lactose or IPTG. RBS: ribosome binding site. IsPETase H104W: This sequence encodes for a mutated IsPETase H104W and is derived from Escherichia coli. pelB: The sequence encodes a signal peptide that enables secretory expression of PETase. 6xHis: A label for protein purification T7 terminator: Terminates transcription. "

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005


[edit]
Categories
Parameters
None