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Part:BBa_K5299205
BBa_J45992 - BBa_B0034 - BBa_I746916 - BBa_B0015
Description
It consists of a promoter, a RBS, a sfGFP and a terminator. The BBa_J45992 is a promoter active in the stationary phase.
Usage and Biology
It is able to produce the sfGFP according to the promoter's abilities.
It was constructed in order to check the strength of the P3.1 promoter. For our experiments, this construct was used as a phase activation control for the P3.1 promoter ( BBa_K4583008 ). Also, it served as a strength control, as they are both active in the stationary phase.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 225
Results
Measurements for OD 600nm and fluorescence (488nm,515nm) were taken over the course of 16 hours, in a 96-well microplate. Clonings were done according to the Golden Braid method, leaving us with level α constructs in the pDGB3a1 backbone BBa_K4213058.
Constructs and pDGB3a1 (empty vector) transformed into E.coli BL21 (DE3) chassis, incubated at 37oC, 180rpm for 16 hours.
Plated 120 ul 5 times, out of each single liquid bacterial culture, created from the same bacterial colony, in order to establish accuracy through technical repeats.
Medium used was M9 due to minimal interference, with D-glucose serving as the carbon source. Also, served as blank, plated 5 times.
Measurements were normalised as such: using the average price of fluorescence for the 5 technical repeats and dividing it by the average price of OD.
Standard deviation included in the graphs.
Measurements were taken over the course of 16 hours. Here are the results for this part (mentioned in the graph as J45992 B0034).
![](https://static.igem.wiki/teams/5299/mar/new-j23119-p3-1-j45992-b0034.png)
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