Composite

Part:BBa_K5206004

Designed by: Yaoyao Liang   Group: iGEM24_Nanjing-BioX   (2024-09-30)
Revision as of 19:34, 30 September 2024 by Registry (Talk | contribs)

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alsR-Palsl-sfGFP

This part consists of BBa_K5206001 and BBa_K5206002. We constructed a composite part by fusing the sequence of BBa_K5206001, encoding the transcriptional regulator AlsR, with the expression vector of BBa_K5206002. PalsI is located in the middle of alsR and sfGFP and controls their expression. The AlsR transcriptional regulatory protein is transcriptionally translated from alsR, and it represses sfGFP expression. When D-allose is present in the environment, AlsR binds preferentially to D-allose and the inhibition will be lifted.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2355
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1640


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