Composite

Part:BBa_K5206003

Designed by: Yaoyao Liang   Group: iGEM24_Nanjing-BioX   (2024-09-30)
Revision as of 19:28, 30 September 2024 by Registry (Talk | contribs)

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Palsl-alsR

This part is composed of BBa_K5206000, BBa_K5206001 and BBa_K731721. BBa_K5206000 (alsR) is derived from Escherichia coli BL21(DE3), which is terminated by BBa_K731721 (T7 terminator). In the E. coli genome, AlsR is responsible for the control of six genes related to D-allose metabolism, aISRBACEK and its bidirectional promoter BBa_K5206001(PalsI). The gene downstream of the bidirectional promoter is also regulated by AlsR. When D-allose is present in the environment, the AslR transcriptional regulatory protein binds preferentially to D-allose and deregulates downstream genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1263
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None