Composite

Part:BBa_K5206002

Designed by: Yaoyao Liang   Group: iGEM24_Nanjing-BioX   (2024-09-30)
Revision as of 18:24, 30 September 2024 by Registry (Talk | contribs)

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Palsl-sfGFP

We placed the green fluorescent protein gene BBa_I746916 (sfGFP) downstream of a bidirectional promoter BBa_K5206000(PalsI) under the control of AlsR to enable real-time fluorescent signal reporting of promoter activity. Subsequently, the plasmid pCDFDuet-1-PalsI-sfGFP was constructed to verify this Part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1092
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 377


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Categories
Parameters
None