Part:BBa_K5322034
Programmed Cell Death 1 Ligand 1 Functional Domain [Mus musculus]
Usage and Biology
The full-length mouse PD-L1 protein has a large molecular weight and a complex structure. Therefore, we referred to the descriptions of mouse PD-L1 in the NCBI database (ADK70951.1, ADK70950.1, Q9NZQ7.1, AAH66841.1) and truncated the Programmed Cell Death 1 Ligand 1 [Mus musculus] (BBa_K5322033). The functional domain—the immunoglobulin variable (IgV) domain—corresponds to residues 20 to 130 in the 290 amino acid sequence. To ensure that the functionality of the truncated protein is preserved, we retained the first 19 amino acids and the last 20 amino acids (131-150), aiming to minimize any impact on the IgV structure.
Protein Structure Prediction
2.1 AlphaFold 2 Prediction Results
For the PD-L1 protein, we utilized AlphaFold 2 from Google DeepMind for protein structure prediction, aiming to gain a deeper understanding of PD-L1.
AlphaFold 2 Prediction Results:
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Figure 2-1 AlphaFold 2 Prediction Results
2.2 AlphaFold 3 Prediction Results
With the release of AlphaFold 3, the accuracy of protein structure prediction has been further improved. Therefore, we conducted another round of protein structure prediction.
AlphaFold 3 Prediction Results:
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Figure 2-2 AlphaFold 3 Prediction Results
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 316
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 316
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 216
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 316
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 316
- 1000COMPATIBLE WITH RFC[1000]
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