Part:BBa_K5293015

pHREAC_eGFP_VAC

pHREAC plasmid backbone for vacuole targeted protein expression with mCherry as a screening tool during cloning

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2344
  Illegal EcoRI site found at 4042
  Illegal XbaI site found at 940
  Illegal SpeI site found at 5888
  Illegal PstI site found at 8545
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2344
  Illegal EcoRI site found at 4042
  Illegal NheI site found at 3253
  Illegal SpeI site found at 5888
  Illegal PstI site found at 8545
  Illegal NotI site found at 5742
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2344
  Illegal EcoRI site found at 4042
  Illegal BglII site found at 1307
  Illegal BglII site found at 2570
  Illegal BglII site found at 2590
  Illegal BglII site found at 9360
  Illegal BamHI site found at 946
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2344
  Illegal EcoRI site found at 4042
  Illegal XbaI site found at 940
  Illegal SpeI site found at 5888
  Illegal PstI site found at 8545
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2344
  Illegal EcoRI site found at 4042
  Illegal XbaI site found at 940
  Illegal SpeI site found at 5888
  Illegal PstI site found at 8545
  Illegal NgoMIV site found at 3054
  Illegal NgoMIV site found at 4212
  Illegal NgoMIV site found at 4738
  Illegal NgoMIV site found at 5614
  Illegal NgoMIV site found at 5738
  Illegal NgoMIV site found at 6765
  Illegal NgoMIV site found at 7356
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.