Plasmid_Backbone
Part:BBa_K5293018
Designed by: Arnaud Boudigou Group: iGEM24_uOttawa (2024-09-23)
pHREAC_eGFP_CYT
pHREAC plasmid backbone for cytoplasm targeted protein expression with mCherry as a screening tool during cloning
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2326
Illegal EcoRI site found at 4024
Illegal XbaI site found at 922
Illegal SpeI site found at 5870
Illegal PstI site found at 8527 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2326
Illegal EcoRI site found at 4024
Illegal NheI site found at 3235
Illegal SpeI site found at 5870
Illegal PstI site found at 8527
Illegal NotI site found at 5724 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2326
Illegal EcoRI site found at 4024
Illegal BglII site found at 1289
Illegal BglII site found at 2552
Illegal BglII site found at 2572
Illegal BglII site found at 9342
Illegal BamHI site found at 928 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2326
Illegal EcoRI site found at 4024
Illegal XbaI site found at 922
Illegal SpeI site found at 5870
Illegal PstI site found at 8527 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2326
Illegal EcoRI site found at 4024
Illegal XbaI site found at 922
Illegal SpeI site found at 5870
Illegal PstI site found at 8527
Illegal NgoMIV site found at 3036
Illegal NgoMIV site found at 4194
Illegal NgoMIV site found at 4720
Illegal NgoMIV site found at 5596
Illegal NgoMIV site found at 5720
Illegal NgoMIV site found at 6747
Illegal NgoMIV site found at 7338 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
[edit]
Categories
Parameters
None |