Part:BBa_K5461001
J23119-BBa_B0034- POI -Spycatcher- BBa_B1006
The New Composite Part BBa_K5461001 functions to form a POI-SpyCatcher fusion protein for linking with the SpyTag, serving as the "plug-and-play functional module" of the bacterial cellulose modification machine. The structure of BBa_K5461001 is J23119-BBa_B0034-POI-SpyCatcher-BBa_B1006. We utilized commonly used iGEM promoter J23119, RBS BBa_B0034, and terminator BBa_B1006. Additionally, we designed standard BsaI interfaces (GGTCTCaGGTAtctagt - POI - GAGCtGAGACCa) on both sides of the POI, allowing for easy replacement of the POI. The POI standard interface in BBa_K5461000 implies the infinite potential for extending the functionalities of the bacterial cellulose modification machine. Future iGEMers can utilize this system to attempt various modifications of bacterial cellulose or even perform high-throughput screening by replacing the POI with a POI library.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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