Coding

Part:BBa_K5322033

Designed by: Xinquan Zhou   Group: iGEM24_NJTech-China   (2024-09-30)
Revision as of 12:05, 30 September 2024 by Registry (Talk | contribs)

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Programmed Cell Death 1 Ligand 1 [Mus musculus]

Based on the descriptions of the mouse PD-L1 protein structure in the NCBI database (ADK70951.1, ADK70950.1, Q9NZQ7.1, AAH66841.1), as well asprogrammed cell death 1 ligand 1 precursor [Mus musculus](NP_068693.1), we optimized the DNA sequence according to the codon usage table for Escherichia coli. The optimized DNA sequence was obtained accordingly.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 295
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 253
    Illegal PstI site found at 295
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 295
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 295
    Illegal NgoMIV site found at 684
    Illegal AgeI site found at 516
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None