Device

Part:BBa_K5477048

Designed by: Kate Escobar, Victor Bay   Group: iGEM24_UCopenhagen   (2024-09-30)
Revision as of 11:06, 30 September 2024 by Registry (Talk | contribs)

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Testing of the SUPERMOM Concept

This system provides the ability to detect and detoxify endocrine-disrupting chemicals (EDCs), with a particular focus on BPA and related compounds, by utilizing a sophisticated combination of ligand detection, signal transduction, and enzymatic detoxification. 

  Ligand Detection: The receptor module (pRET2-LexA-mERα(LBD)) contains a mutant Estrogen Receptor Alpha (mERα), specifically modified to detect and bind to BPA and similar ligands. Once BPA binds, the receptor undergoes a conformational change, enabling transcriptional activation of the downstream modules.

  Signal Transduction: The activated LexA-mERα(LBD) fusion protein then binds to the Lex6Op operator sequences in the reporter module (Lex6Op-pLEU2-NanoLuc), triggering the expression of NanoLuc luciferase, which produces a bioluminescent signal. This signal provides a quantitative and real-time indication of ligand binding and detection.

  Detoxification: Concurrently, the Lex6Op sequences also control the detoxification module (CYP3A4-MYC-pGAL1/10-POR), which is responsible for metabolizing BPA or related EDCs. CYP3A4, in conjunction with POR (NADPH-cytochrome P450 reductase), functions to oxidize and detoxify the harmful compounds, converting them into less toxic or more readily excretable metabolites.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1606
    Illegal XbaI site found at 4766
    Illegal PstI site found at 1790
    Illegal PstI site found at 1961
    Illegal PstI site found at 5712
    Illegal PstI site found at 5909
    Illegal PstI site found at 6293
    Illegal PstI site found at 6733
    Illegal PstI site found at 6793
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 182
    Illegal PstI site found at 1790
    Illegal PstI site found at 1961
    Illegal PstI site found at 5712
    Illegal PstI site found at 5909
    Illegal PstI site found at 6293
    Illegal PstI site found at 6733
    Illegal PstI site found at 6793
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1740
    Illegal BglII site found at 2751
    Illegal XhoI site found at 4823
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1606
    Illegal XbaI site found at 4766
    Illegal PstI site found at 1790
    Illegal PstI site found at 1961
    Illegal PstI site found at 5712
    Illegal PstI site found at 5909
    Illegal PstI site found at 6293
    Illegal PstI site found at 6733
    Illegal PstI site found at 6793
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1606
    Illegal XbaI site found at 4766
    Illegal PstI site found at 1790
    Illegal PstI site found at 1961
    Illegal PstI site found at 5712
    Illegal PstI site found at 5909
    Illegal PstI site found at 6293
    Illegal PstI site found at 6733
    Illegal PstI site found at 6793
    Illegal NgoMIV site found at 6115
    Illegal NgoMIV site found at 6234
    Illegal AgeI site found at 37
  • 1000
    COMPATIBLE WITH RFC[1000]


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