Coding

Part:BBa_K5301004

Designed by: Xiaowen Sun   Group: iGEM24_BNU-China   (2024-09-22)
Revision as of 07:08, 22 September 2024 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


SpyCatcher can achieve covalent binding of proteins through Tag-Catcher interaction.

SpyCatcher comes from the spontaneous isopeptide bond domain in streptococcus pyogenes fibronectin-binding protein FbaB. It can cooperate with SpyTag to achieve covalent binding of proteins through Tag-Catcher interaction. The robust reaction conditions and irreversible linkage of SpyTag-Catcher provide a targetable lock in cells and a stable module for new protein architectures.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None