Composite

Part:BBa_K5322000:Design

Designed by: Yingying Yu   Group: iGEM24_NJTech-China   (2024-09-27)
Revision as of 11:02, 30 September 2024 by Casey (Talk | contribs) (→‎Design Notes)


Constitutive Mfp3 Expression System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 210
    Illegal NotI site found at 174
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To address the reduced mucus layer thickness and dysbiosis causing inflammation in patients with enteritis, we aim to resolve the dysregulation of the mucosal system from the ground up. A natural high-adhesion protein from marine mussels—mussel foot protein (Mfp)—has garnered our attention, particularly Mfp3, which is one of the proteins with the highest DOPA content among the six mussel proteins. We utilized Escherichia coli BL21(DE3) to establish a production system for Mfp, enhancing adhesion in damaged intestines and regulating gut microbiota. Consequently, we designed the plasmid pET29a-J23119-RBS-Mfp3-T7.

Source

123456

References