Composite

Part:BBa_K5453005

Designed by: Peipei Liu   Group: iGEM24_SZSAIS-Shenzhen   (2024-09-18)
Revision as of 15:19, 18 September 2024 by Registry (Talk | contribs)

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pBAD-Gatz-PGP-Trrnb

Gatz and PGP were successively ligated under the promoter of pBAD, and the expression of the corresponding proteins was induced after the addition of arabinogalactan inducer, so as to construct the Tagatose synthesis pathway in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 658
    Illegal NgoMIV site found at 784
    Illegal AgeI site found at 329
    Illegal AgeI site found at 1556
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/biosynthesis
Parameters
function