Primer

Part:BBa_K5079013

Designed by: Marianna Florez   Group: iGEM24_Queens-Canada   (2024-09-28)
Revision as of 08:50, 30 September 2024 by Jasonsong (Talk | contribs)


K45_CP_P95 Switch primers into pET-28a Switch 7 (Fwd)

This is the forward primer for Part:BBa_K5079032. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. The sequence is: ACTTTAAGAAGGAGATATAC atgtcgggaaatcccttgg. 3’ GC Content is 53%, 3' length is 19nt and 3' Tm is 66°C.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None