Composite

Part:BBa_K5269023

Designed by: Yuichiro Nakai   Group: iGEM24_TokyoTech   (2024-09-28)
Revision as of 03:47, 29 September 2024 by Nakaiyuichiro (Talk | contribs)


SpyTag-Mgfp-5-His-Tag and BsmB I cut site(for GGA)

It contains SD-sequences(BBa_K5269034), SpyTag, Mgfp-5 (BBa_5269013) and His-Tag sequences. In addition, the BsmB I cleavage sites at both ends of the sequence allow for Golden Gate Assembly to the Golden Gate Assembly Site (BBa_K5269020) and T7 promoter-shRNA-T7 terminator-Golden Gate Assembly Site(BBa_K5269025). The SpyCatcher-SpyTag system works when used with Lpp-ompA-SpyCatcher(BBa_K5269026) or INPNC-SpyCatcher(BBa_K5269027). Therefore, we expect the adhesion protein Mgfp-5 to be stable on the Membrane Vesicles, which will facilitate the retention of our shRNA-encapsulated membrane vesicles on the plant surface.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 381
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None