Signalling

Part:BBa_K212006

Designed by: Elias Scheer   Group: iGEM09_Brown   (2009-10-20)
Revision as of 23:12, 21 October 2009 by Escheer (Talk | contribs)

mRBP (modified ribose binding protein - now binds histamine)

This is a mutated version of RBP, an E. coli periplasmic ribose-binding protein. Natively, ribose binds as a ligand, transducing a message to the trg domain of a transmembrane histidine kinase. Here we have computationally redesigned RBP's ligand binding pocket to bind histamine preferentially over ribose, yielding mRBP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None