Composite

Part:BBa_K5089011:Design

Designed by: Camille Prefontaine   Group: iGEM24_UManitoba   (2024-09-29)
Revision as of 23:11, 29 September 2024 by Registry (Talk | contribs)

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ABO(H243R)-CsgA-LCI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 812
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 831
    Illegal NgoMIV site found at 977
    Illegal AgeI site found at 335
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This expression system is codon optimized for expression in E.coli


Source

ABO(H243R) is an α/β hydrolase mutant with a H243R mutation to improve enzyme stability at acidic pH. Wildtype enzyme is native to Alcanivorax borkumensis, gene number 2449 [1]. LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene, polypropylene and polylactic acid [2]. CsgA is a protein component of E.coil biofilms, which can be used as an anchor to surface display proteins [3].

References