Coding

Part:BBa_K5324001:Design

Designed by: Sebastian Nolte   Group: iGEM24_Alma   (2024-09-26)
Revision as of 16:38, 26 September 2024 by Jacksonhall (Talk | contribs) (→‎Design Notes)


Destructin-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 55
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 832
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was codon optimized for expression in E.coli. Bsal and Bsml restriction cut sites removed with 1 bp mutations.

Source

Genomic sequence obtained from GeneBank. Originated from P. destructans.

References

Bennett, R. J. (2015, April 15). Destructin-1 is a collagen-degrading endopeptidase ... Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome. https://www.pnas.org/doi/full/10.1073/pnas.1507082112 U.S. National Library of Medicine. (n.d.). RecName: Full=subtilisin-like protease 2; AltName: Full=destructin-1;. National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/protein/L8FSM5.1