Coding
Part:BBa_K5089009:Design
Designed by: Camille Prefontaine Group: iGEM24_UManitoba (2024-09-29)
ABO2449(H243R)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 735
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 754
Illegal NgoMIV site found at 900
Illegal AgeI site found at 258 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence for this part is codon-optimized for expression in E. coli.
Source
Alcanivorax borkumensis, gene number 2449