Composite

Part:BBa_K5089006:Design

Designed by: Camille Prefontaine   Group: iGEM24_UManitoba   (2024-09-29)
Revision as of 20:29, 29 September 2024 by Registry (Talk | contribs)

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CLE-CsgA-LCI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 226
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This expression system is codon optimized for expression in E.coli.


Source

CLE is a cutinase from Cryptococcus sp. strain S-2, previously shown to cleave carbon-chain ester substrates [1]. LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene, polypropylene and polylactic acid [2]. CsgA is a protein component of E.coli biofilms, which can be used as an anchor to surface display proteins [3].

References