Coding

Part:BBa_K5117010:Design

Designed by: Jenny Sauermann, Lilli Kratzer, Katrin Lehmann   Group: iGEM24_TU-Dresden   (2024-08-31)
Revision as of 17:20, 28 September 2024 by Jesa98 (Talk | contribs)


BhrPET


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 579
    Illegal AgeI site found at 624
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

removal of restriction sites which are used for RFC10 or TypeIIS standards, addition of a signal peptide SPaprE for secretion in Bacillus subtilis, addition of start codon (ATG) and stop codon (TAA)

Source

Bacterium HR29