Translational_Unit

Part:BBa_K5127006:Design

Designed by: Yinuo Li   Group: iGEM24_BNDS-China   (2024-09-29)
Revision as of 17:54, 29 September 2024 by Enolyn1214 (Talk | contribs)


iadCDE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2355
    Illegal PstI site found at 2172
    Illegal PstI site found at 2750
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2355
    Illegal NheI site found at 677
    Illegal PstI site found at 2172
    Illegal PstI site found at 2750
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2355
    Illegal BglII site found at 2364
    Illegal BglII site found at 2416
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2355
    Illegal PstI site found at 2172
    Illegal PstI site found at 2750
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2355
    Illegal PstI site found at 2172
    Illegal PstI site found at 2750
    Illegal NgoMIV site found at 623
    Illegal NgoMIV site found at 1614
    Illegal AgeI site found at 387
    Illegal AgeI site found at 1416
    Illegal AgeI site found at 1857
    Illegal AgeI site found at 2280
    Illegal AgeI site found at 2714
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli.


Source

Genome of Variovorax species

References

Conway, J. M., Walton, W. G., Salas-González, I., Law, T. F., Lindberg, C. A., Crook, L. E., Kosina, S. M., Fitzpatrick, C. R., Lietzan, A. D., Northen, T. R., Jones, C. D., Finkel, O. M., Redinbo, M. R., & Dangl, J. L. (2022). Diverse MarR bacterial regulators of auxin catabolism in the plant microbiome. Nature Microbiology, 7(11), 1817–1833. https://doi.org/10.1038/s41564-022-01244-3