Part:BBa_K5035005:Design
A0A2E5NSL8 reductive dehalogenase
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 901
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 901
Illegal NheI site found at 184 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 901
Illegal BglII site found at 354 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 901
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 901
Illegal AgeI site found at 661 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
A hexahistidine tag for nickel column purification was added to A0A2E5NSL8.
Source
ATGCATCATCATCATCATCATATCCATCCGCGTGGCTCCGAACGTCCAGACCGTCGTGGCGCTCTGTTCGCTGGTTCTCCGTCCGATAGCTGTGACGCGGGTCCTTCTGGCGTAGACGGCCCAGCTTGGGAAGTTCTGGAAAGCTTTGAACGCTTCAACCAGGTTGACGACGTATTCAACCGTGCTAGCTGGGACCCAGAAGTGGCAACCGAGGAAGCTCGCCGCTTTTTTGCGTCTTATCGTGAACCGCTGCGCTCCTGGCGTAACGCAAGCGGCTTCGGCCAGCGTGATTATGCAATTCGCAACGCCGCTTGGCAAGTTGTAGATGTCTTCGCAGAGATGCACGACGCTGAAGATCTGCGTGACGGTTTTCTGAGCCCGCTGTCTCAACTGCGTGATGGCCCGTCTGAACGCTTTGATCTGGGTTCTCCGGAAGAAGCTTCTGCTACGGTGAAACAGGTTGCACGTACCTTCGGCGCTGACCTGGTTGGTCTGTGCGCGCACGATGAACGCTGGGTGTACACCGAACGTTACGCGCGTCGTGCGGATGAAGGTCGTCCAGTCGGCCTGCCGGATGGTCTGACCAGCGTGGTTGTCATTGGTCAGGCTATGGACCGTGGCCTGGTTGATACCGTACCGTCCGCACTGTCCGGTGCCGCTACCGGTCTGGGTTACTCCAAAGACGCTCTGGTCCTGCTGGCAACGGCACAGTGGCTGCGTAACCTGGGTTATCGCGCAGTTCCTTCTCTGAACGACACGGCGCTGGCTATCCCGCTGGCAATTCAAGCAGGTCTGGGTGAATACGGCCGCCACGGCATGGTAATCACCCCGGAATTTGGCCCGCGTGTACGTTTCGGCAAAATCTTCACCGATCTGCCTGTGGCCCACGACCAGCCTATCGAATTCGGTGTTACTCGCTTCTGCGAGCAGTGTCGCCGTTGCTCCAACGCATGCCCGCCAGGTGCAATTCCGGACGCAGGCCCGGTGGCGGTAAAACTGAACCGCAGCAGCATCGGTGGCGTTCGTAAGTGGTCCGTGGACGGCGAAAAATGCTTCAGCTACTGGGCTCAGATCGCGAACGACTGCATGATCTGTATGCGCGTCTGTCCGTACAACAAAGACTTTTCCCGTTTCCGTCACCGTCTGGGTCGTCGTCTGGCTGCTACGCGTCTGCGCGGTATGATGCTGTGGCTGGAAGAACGTATGGGTTACGGCAAGCGCCTGCGTCCACGTGACTGGTGGAAAGGTCGTGACGATCGTCCGACCCTACTCTGGCGTCGATGA