Composite

Part:BBa_K5267041

Designed by: Hanyue Mao   Group: iGEM24_NUDT-CHINA   (2024-09-29)
Revision as of 16:24, 29 September 2024 by Registry (Talk | contribs)

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P_5xCRE>IgK->Nluc->bGH_polyA

When melatonin binds to the MT1 receptor, the activation of adenylate cyclase (AC) is activated, regulating the level of the second messenger cAMP, while activating protein kinase to further amplify the signal. It catalyzes the phosphorylation of CREB in the nucleus and regulates the expression of downstream genes.Then the cell will glow blue fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 103
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 376
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None