Composite

Part:BBa_K5109023:Design

Designed by: Lisa Faccincani   Group: iGEM24_Uni-Padua-IT   (2024-09-28)
Revision as of 06:52, 28 September 2024 by Registry (Talk | contribs)

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Deha2 surface display system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 639
    Illegal BamHI site found at 1372
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 667


Design Notes

We decided to exploit the first codon of the coding sequence to introduce NdeI cutting site, which is CATATG.

To better implement the design solution described, we modified the restriction enzyme sites compared to BBa K5109001: we removed the two BsaI cut sites downstream of the passenger, because at the same time, we introduced a BsaI restriction site upstream of the sequence encoding the passenger, allowing for more elegant manipulation of the surface expression cassette: with these improvements, it is now possible to change the passenger protein whether the carrier is present or removed from the expression cassette.



Source

This part is form from the assebly of different basic parts: it was designed on Benchling and subsequently synthesized.

References