Generator

Part:BBa_K5074001

Designed by: Wang Zishuo   Group: iGEM24_ICJFLS   (2024-09-29)
Revision as of 15:15, 29 September 2024 by Registry (Talk | contribs)

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AmilCP generator controlled by Shigella RNA aptamer toehold switch

It comprises Shigella dysenteriae RNA aptamer, RBS, Linker and reporter gene amilCP. At the presence of Shigella dysenteriae, it binds to its RNA aptamer, opening the toehold switch to trigger the expression of amilCP, which is easily deteced. It is used to detect the bacteria Shigella in refrigerator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None