Coding
creA

Part:BBa_K5108003

Designed by: Léa Breton   Group: iGEM24_Toulouse-INSA-UPS   (2024-08-28)
Revision as of 13:10, 29 September 2024 by Yohannquivet (Talk | contribs)

Creatinase from Pseudomonas putida


    Contents
  1. Usage and Biology
  2. References



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 225
    Illegal BsaI site found at 1012

Usage and Biology

CreA is an enzyme that catalyzes the degradation of creatine into sarcosine in Pseudomonas putida. In the context of our project, the gene encoding this enzyme (creA) was synthesized in an operon with the gene for creatine degradation. You can find more information about our construct and our results in BBa_K5108009.


References

  1. UniProt. (s. d.-b). https://www.uniprot.org/uniprotkb/P38488/entry
  2. Cloning, Expression and Purification of Pseudomonas putida ATCC12633 Creatinase. (2017, 1 décembre). PubMed. https://pubmed.ncbi.nlm.nih.gov/29090065/

[edit]
Categories
//cds/enzyme
Parameters
biologyPseudomonas putida
proteinCreatinase
uniprotP38488