Part:BBa_K5108004

Creatinine amidohydrolase form Pseudomonas putida

Contents
1. Usage and Biology
2. References

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 61
  Illegal NgoMIV site found at 663
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 199
  Illegal BsaI.rc site found at 547
  

Usage and Biology

CrnA is an enzyme that catalyzes the degradation of creatinine into creatine in Pseudomonas putida. In the context of our project, the gene encoding this enzyme (crnA) was synthesized in an operon with the gene for creatine degradation. You can find more information about our construct and our results in BBa_K5108009.

References

1. UniProt. (s. d.). https://www.uniprot.org/uniprotkb/P83772/entry
2. Tsuru, D., Oka, I., & Yoshimoto, T. (1976c). Creatinine Decomposing Enzymes inPseudomonas putida. Agricultural And Biological Chemistry, 40(5), 1011‑1018. https://doi.org/10.1080/00021369.1976.10862151