Coding

Part:BBa_K211002:Design

Designed by: ZHONG Wanting   Group: iGEM09_HKUST   (2009-10-13)
Revision as of 21:59, 21 October 2009 by Chuchu (Talk | contribs) (→‎Design Notes)

RI7-odr10 chimeric GPCR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 761
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed with PCR. Primer design.jpg Primer Sequence * P1 RI7-N F ATATCTGAATTCGCCACCATGGAACGAAGGAACCAC

P2 RI7-C R GTATACGCGGCCGCACCAATTTTGCTGCCTTTG

P3 RI7-N R GAGAAGAA ATACATGGGTTTGTGGAG

P4 Odr-10 F CCATGTAT TTCTTCTCAGTATTCTCG

P5 Odr-10 R GCAGTA AATGATCAGAATCAGAATGAG

P6 RI7-C F GATCATT TACTGCTTGCGCAACC


BioBrick F ATATCT TCTAGATGGAACGAAGGAACCAC BioBrick R GTATACACTAGTA TTA TTA ACCAATTTTGCTGCC


We would like to thank Professor Piali Sengupta, Department of Biology, Brandeis University for the kind gift of the odr-10 cDNA, and Professor Stuart Firestein, Department of Biological Sciences, Columbia University for the kind gift of the rat I7 olfactory receptor cDNA.

Source

Caenorhabditis elegans olfactory receptor Odr-10 (GenBank: U49449.1)

Rat olfactory protein, clone I7 (GenBank: M64386.1)

References

Radhika, V. et al . 2007. Chemical sensing of DNT by engineered olfactory yeast strain. Nat. Chem. Biol. 3 : 325–330