RBS

Part:BBa_K5108007

Designed by: Léa Breton   Group: iGEM24_Toulouse-INSA-UPS   (2024-08-29)
Revision as of 10:05, 29 September 2024 by Yohannquivet (Talk | contribs)

hcnA RBS from Pseudomonas protegens (CHA0)


    Contents
  1. Usage and Biology
  2. References



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Ribosome Binding Site of gene hcnA of P. fluorescens. It was used for protein translation in P. fluorescens from [1]. This RBS was synthesized in an operon with two genes and another RBS and cloned into a plasmid. After transformation in P. fluorescens, our team had successful protein expression with this RBS. Check out composite part BBa_K5108009for more information.


References

  1. Blumer, C., Heeb, S., Pessi, G., & Haas, D. (1999). Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proceedings Of The National Academy Of Sciences, 96(24), 14073‑14078. https://doi.org/10.1073/pnas.96.24.14073
    2.

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Categories
Parameters
biologyPseudomonas protogens
rbs