Coding
crnA

Part:BBa_K5108004:Design

Designed by: Léa Breton   Group: iGEM24_Toulouse-INSA-UPS   (2024-08-28)
Revision as of 12:58, 29 September 2024 by Yohannquivet (Talk | contribs)


hcnA RBS from Pseudomonas aeruginosa (PAO1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Sources

The codon optimized nucleotidic sequence from Pseudomonas putida adapted to Pseudomonas putida is obtained thanks to the Codon Optimization Tool from Integrated DNA Technologies codon optimizer tool.. This step permits to lower complexity and minimize secondary structures. The Part was obtained through IDT gBlock synthesis

References

  • Blumer, C., Heeb, S., Pessi, G., & Haas, D. (1999). Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proceedings Of The National Academy Of Sciences, 96(24), 14073‑14078. https://doi.org/10.1073/pnas.96.24.14073
  • Blumer, C., Heeb, S., Pessi, G., & Haas, D. (1999). Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proceedings Of The National Academy Of Sciences, 96(24), 14073‑14078. https://doi.org/10.1073/pnas.96.24.14073