Part:BBa_K5382120
InaK-linker-Im7_Cell membrane anchoring protein and immune protein complex
InaK is a kind of ice nucleoprotein of Pseudomonas syringae KCTC1832. Ice nucleoprotein is a kind of protein that can promote the formation of ice crystals in organisms. Its N-terminal domain can be tightly anchored to the surface of E. coli cells as an anchor moda, so as to enable surface display. linker here is a short peptide sequence used to link InaK and Im7. Im7 is an immune protein that has a high affinity with CL7(an engineered variant of colicin CE7).Through their high affinity, InaK-linker-Im7 can be tightly bound to CL7-Linker-SFGFP (another composite part we registered) and InaK in CL7-Linker-SFGFP can be tightly anchored to the cell membrane, thus anchoring the entire system to the membrane surface. Finally, the Inak-linker-Im7-CL7-linker-sfGFP system is displayed on the surface of the membrane.sfGFP is a green fluorescent protein. In this system, sfGFP can verify the success of anchoring, and then it can be replaced with single-chain antibodies, nano-antibodies, etc., to play a targeting role. It should be noted that the system we designed uses the high affinity of CL7 and Im7, so some components can be flexibly replaced according to needs, and are suitable for different use scenarios according to different needs. For example, according to different cells to replace the ice nucleated protein, according to different targeting targets to replace sfGFP with different single-chain antibodies, nano antibodies and so on.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1800
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 722
Illegal NgoMIV site found at 962
Illegal NgoMIV site found at 1130
Illegal AgeI site found at 517 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 692
Illegal BsaI.rc site found at 1182
Illegal SapI.rc site found at 326
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