Regulatory

Part:BBa_K5332001:Design

Designed by: Qilin Yu   Group: iGEM24_NKU-China   (2024-09-21)
Revision as of 16:40, 21 September 2024 by Registry (Talk | contribs)

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Reactive oxygen species promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 182
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 182
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 182
    Illegal BglII site found at 253
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 182
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 182
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing, our primary consideration is whether this part of the selected promoter sequence has the same expression transcriptional effect as the original gene sequence. In addition, some of the selected sequences should still have a high affinity with the oxyR regulator of the upstream ROS response pathway.


Source

From the promoter sequence of katG.

References